Phosphorolytic Cleavage of Fructose-6-phosphate by Fructose-6-phosphate Phosphoketolase from Acetobacter xylinum

Phosphorolytic cleavage of fructose-6-phosphate by fructose-6-phosphate phosphoketolase from Acetobacter xylinum.

The synthesis of cellulose by washed suspensions of Acetobatter xylinum proceeds in the presence of iodoacetate at concentrations which completely inhibit the oxidation of glyceraldehyde-a-phosphate (1). Since glucose was the only carbon compound added, the possibility was considered that the energy required for the biosynthetic process may be derived from reactions linked to the pentose phosph...

Phosphorolytic Cleavage of Fructose-&phosphate by Fructose-6-phosphate Phosphoketolase from Acetobacter xylinum*

The synthesis of cellulose by washed suspensions of Acetobatter xylinum proceeds in the presence of iodoacetate at concentrations which completely inhibit the oxidation of glyceraldehyde-a-phosphate (1). Since glucose was the only carbon compound added, the possibility was considered that the energy required for the biosynthetic process may be derived from reactions linked to the pentose phosph...

The determination of fructose-6-phosphate and fructose-1,6-diphosphate.

Characterization of the D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase gene (xfp) from Bifidobacterium lactis.

A D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase (Xfp) from the probiotic Bifidobacterium lactis was purified to homogeneity. The specific activity of the purified enzyme with D-fructose 6-phosphate as a substrate is 4.28 Units per mg of enzyme. K(m) values for D-xylulose 5-phosphate and D-fructose 6-phosphate are 45 and 10 mM, respectively. The native enzyme has a molecular mass...

Study of the fructose 6-phosphate/fructose 1,6-bi-phosphate cycle in the liver in vivo.

1. The method proposed by Rognstad & Katz [(1976) Arch, Biochem, Biophys, 177, 337-345] for the determination of the fructose 6-phosphate/fructose 1,6-bisphosphate cycle by the randomization of carbon between C-1 and C-6 of glucose glucose formed from [1-14C] galactose was applied to anaesthetized rats and conscious mice. 2. It was checked that the hydrolysis of fructose 6-phosphate by glucose ...